BRCAS

Biodiversity Research Center, Academia Sinica

Dr. Wen-Hsiung Li Distinguished Research Fellow

Lab PI

Dr. Wen-Hsiung Li

Distinguished Research Fellow

Brown University, Providence, RI, USA Ph.D.

[ This email address is being protected from spambots. You need JavaScript enabled to view it. ]

Office: terdisciplinary Research Building for Science and Technology  A203

Tel: +886-2-2787-2256

Research Fields
  • Evolutionary Genomics
  • Molecular Evolution
  • Bioinformatics and Computational Biology
Research Directions
    • I. Avian evolutionary genetics and genomics
      Explaining how and why the endless forms of life surrounding us evolved has been a central question in biology.  The feathers of birds display a great degree of diversity among species and body parts.  Our goals are to understand the genetic and genomic changes creating the origin and evolution of these novelties.  We use comparative genomic, transcriptomic, and developmental genetic approaches to gain a deeper understanding of the molecular pathways that control feather development and evolution.
    • II. Synthetic biology: a new approach to biotechnology
      Synthetic biology is a field of translational research that combines science and technology. It has been dubbed a possible “biological” industrial revolution. It is the design and assembly of bio-bricks into genetic circuits for new biological entities and systems. It has a huge potential for applications, e.g., bio-fuel, green materials, agriculture and medicine. It is expected to be a key area in the commercialization of research, with close ties between academics and industry.
    • III. C4 plant leave developmental evolution
      Compared to C3 plants (e.g. rice), C4 plants (e.g., maize) are more efficient in CO2 concentration, and in water and nitrogen use, leading to better adaptation to hotter and dryer environments. Inserting the C4 photosynthetic pathway into rice should increase rice yield, double water use efficiency, and use less fertilizer. Essential to this endeavor is to understand the regulation of kranz anatomy development in C4 plants. We have therefore been using a combination of genomic, bioinformatic and molecular biology approaches to understand the developmental differences between rice and maize and to identify the key regulators of kranz anatomy development. We have also been transforming C4 genes into the rice genome.

 

Latest published paper

期刊論文 ( 2017 - 2020 )

  1. Lee MH, Hsu TL, Lin JJ, Lin YJ, Kao YY, Chang JJ, Li WH*, 2020, “Constructing a human complex type N-linked glycosylation pathway in Kluyveromyces marxianus.”, PloS one, 15(5), e0233492. (SCIE) (IF: 2.766; SCIE ranking: 23.4%) Link
  2. Anandharaj M, Lin YJ, Rani RP, Nadendla EK, Ho MC, Huang CC, Cheng JF, Chang JJ, Li WH*, 2020, “Constructing a yeast to express the largest cellulosome complex on the cell surface.”, Proceedings of the National Academy of Sciences of the United States of America, 117(5), 2385-2394. (SCIE) (IF: 9.504; SCIE ranking: 7.8%) Link
  3. Wang WF, Lu MJ, Cheng TR, Tang YC, Teng YC, Hwa TY, Chen YH, Li MY, Wu MH, Chuang PC, Jou R, Wong CH, Li WH*, 2020, “Genomic Analysis of Mycobacterium tuberculosis Isolates and Construction of a Beijing Lineage Reference Genome.”, Genome biology and evolution, 12(2), 3890-3905. (SCIE) (IF: 3.94; SCIE ranking: 28.6%,24.6%) Link
  4. Liu HL, Chang JJ, Thia C, Lin YJ, Lo SC, Huang CC, Li WH*, 2019, “Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories.”, Microbial cell factories, 18(1), 155. (SCIE) (IF: 3.831; SCIE ranking: 22.4%) Link
  5. Wang L, Zhao L, Zhang X, Zhang Q, Jia Y, Wang G, Li S, Tian D, Li WH*, Yang S, 2019, “Large-scale identification and functional analysis of NLR genes in blast resistance in the Tetep rice genome sequence.”, Proceedings of the National Academy of Sciences of the United States of America, 116(37), 18479-18487. (SCIE) (IF: 9.504; SCIE ranking: 7.8%) Link
  6. Gomez Luciano LB, Jason Tsai I, Chuma I, Tosa Y, Chen YH, Li JY, Li MY, Jade Lu MY, Nakayashiki H, Li WH*, 2019, “Blast fungal genomes show frequent chromosomal changes, gene gains and losses, and effector gene turnover.”, Molecular biology and evolution, 36(6),1148-1161. (SCIE) (IF: 6.202; SCIE ranking: 12.8%,9%,10.4%) Link
  7. Chang YM, Lin HH, Liu WY, Yu CP, Chen HJ, Wartini PP, Kao YY, Wu YH, Lin JJ, Lu MJ, Tu SL, Wu SH, Shiu SH, Ku MSB, Li WH*, 2019, “Comparative transcriptomics method to infer gene coexpression networks and its applications to maize and rice leaf transcriptomes.”, Proceedings of the National Academy of Sciences of the United States of America, 116(8), 3091-3099. (SCIE) (IF: 9.504; SCIE ranking: 7.8%) Link
  8. Lin JJ, Bhattacharjee MJ, Yu CP, Tseng YY, Li WH*, 2019, “Many human RNA viruses show extraordinarily stringent selective constraints on protein evolution.”, Proceedings of the National Academy of Sciences of the United States of America, 116(38), 19009-19018. (SCIE) (IF: 9.504; SCIE ranking: 7.8%) Link
  9. Huang CJ, Lu MY, Chang YW, Li WH*, 2018, “Experimental Evolution of Yeast for High Temperature Tolerance.”, Molecular biology and evolution, 35(8), 1823-1839. (SCIE) (IF: 6.202; SCIE ranking: 12.8%,9%,10.4%) Link
  10. Huang J, Li J, Zhou J, Wang L, Yang S, Hurst LD, Li WH, Tian D, 2018, “Identifying a large number of high-yield genes in rice by pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 gene knockout.”, Proceedings of the National Academy of Sciences of the United States of America, 115(32), E7559-E7567. (SCIE) (IF: 9.661; SCIE ranking: 6.3%) Link
  11. Pi HW, Anandharaj M, Kao YY, Lin YJ, Chang JJ, Li WH, 2018, “Engineering the oleaginous red yeast Rhodotorula glutinis for simultaneous beta-carotene and cellulase production.”, Scientific reports, 8(1), 10850. (SCIE) (IF: 4.259; SCIE ranking: 15.6%) Link
  12. Chang JJ, Anandharaj M, Ho CY, Tsuge K, Tsai TY, Ke HM, Lin YJ, Ha Tran MD, Li WH*, Huang CC, 2018, “Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis.”, Biotechnology for biofuels, 11, 157. (SCIE) (IF: 5.203; SCIE ranking: 12%,11.9%) Link
  13. Lee MH, Lin JJ, Lin YJ, Chang JJ, Ke HM, Fan WL, Wang TY, Li WH*, 2018, “Genome-wide prediction of CRISPR/Cas9 targets in Kluyveromyces marxianus and its application to obtain a stable haploid strain.”, Scientific reports, 8(1), 7305. (SCIE) (IF: 4.259; SCIE ranking: 15.6%) Link
  14. Chang JJ, Lin YJ, Lay CH, Thia C, Wu YC, Hou YH, Huang CC, Li WH, 2018, “Constructing a cellulosic yeast host with an efficient cellulase cocktail.”, Biotechnology and bioengineering, 115(3), 751-761. (SCIE) (IF: 4.481; SCIE ranking: 14.4%) Link
  15. Wu P, Yan J, Lai YC, Ng CS, Li A, Jiang X, Elsey RM, Widelitz R, Bajpai R, Li WH, Chuong CM, 2018, “Multiple Regulatory Modules Are Required for Scale-to-Feather Conversion.”, Molecular biology and evolution, 35(2), 417-430. (SCIE) (IF: 6.202; SCIE ranking: 12.8%,9%,10.4%) Link
  16. Ke HM, Prachumwat A, Yu CP, Yang YT, Promsri V, Liu KF, Lo CF, Lu MYJ, Lai MC, Tsai IJ, Li WH*, 2017, “Comparative genomics of Vibrio campbellii strains and core species of the Vibrio Harveyi clade”, Scientific Reports, 7, 41394. (SCIE) (IF: 4.259; SCIE ranking: 15.6%) Link
  17. Su SC, Lin CW, Liu YF, Fan WL, Chen MK, Yu CP, Yang WE, Su CW, Chuang CY, Li WH, Chung WH, Yang SF, 2017, “Exome Sequencing of Oral Squamous Cell Carcinoma Reveals Molecular Subgroups and Novel Therapeutic Opportunities.”, Theranostics, 7(5), 1088-1099. (SCIE) (IF: 8.766; SCIE ranking: 6.3%) Link
  18. Chang JJ, Lin YJ, Lay CH, Thia C, Wu YC, Hou YH, Li WH*, 2017, “Constructing a Cellulosic Yeast Host with an Efficient Cellulase Cocktail”, BIOTECHNOLOGY AND BIOENGINEERING, 115(3), 751-761. (SCIE) (IF: 4.481; SCIE ranking: 14.4%) Link
  19. Huang CF, Yu CP, Wu YH, Lu MJ, Tu SL, Wu SH, Shiu SH, Ku MSB, Li WH*, 2017, “Elevated auxin biosynthesis and transport underlie high vein density in C4 leaves.”, Proceedings of the National Academy of Sciences of the United States of America, 114(33), E6884-E6891. (SCIE) (IF: 9.661; SCIE ranking: 6.3%) Link
  20. Chen CK, Yu CP, Li SC, Wu SM, Lu MJ, Chen YH, Chen DR, Ng CS, Ting CT, Li WH*, 2017, “Identification and evolutionary analysis of long non-coding RNAs in zebra finch.”, BMC genomics, 18(1), 117. (SCIE) (IF: 3.729; SCIE ranking: 21.9%,29.9%) Link
  21. Lin YJ, Chang JJ, Lin HY, Thia C, Kao YY, Huang CC, Li WH*, 2017, “Metabolic engineering a yeast to produce astaxanthin.”, Bioresource technology, 245(Pt A), 899-905. (SCIE) (IF: 5.651; SCIE ranking: 8.8%,7.1%,9.8%) Link
  22. Wu P, Yan J, Lai YC, Ng CS, Li A, Jiang X, Lin JJ, Elsey R, Widelitz R, Bajpai R, Li WH, Chuong CM, 2017, “Multiple regulatory modules are required for scale-to-feather conversion”, MOLECULAR BIOLOGY AND EVOLUTION, 35(2), 417-430. (SCIE) (IF: 6.202; SCIE ranking: 12.8%,9%,10.4%) Link
  23. Lin JJ, Wang FY, Li WH*, Wang TY, 2017, “The rises and falls of opsin genes in 59 ray-finned fish genomes and their implications for environmental adaptation.”, Scientific reports, 7(1), 15568. (SCIE) (IF: 4.259; SCIE ranking: 15.6%) Link