(Microbial Diversity and Bioinformatics)
Time：2023. 03. 03 Fri. 15:00
Venue: Auditorium, 1st Floor, Interdisciplinary Research Building
Speaker：Prof. Sung-Keun Rhee
Department of Microbiology, Chungbuk National University, Korea
Title：Unexpected Metabolic Versatility of Aerobic Methanotrophs
Host：Dr. Yin-Ru Chiang
Atmospheric methane (CH4) is a potent greenhouse gas responsible for about 15% of the total greenhouse effect. The amount of CH4 in Earth's atmosphere is continually increasing. The diffusive flux of CH4 is oxidized by aerobic methanotrophic bacteria at oxic-anoxic interface in terrestrial environments, thereby reducing CH4 emission. Methanotrophs were long assumed to have a limited substrate spectrum, including methane, methanol, and occasionally other C1 compounds, but no substrates containing carbon–carbon bonds.
To date, there has been a clear distinction between thiotrophic and methanotrophic microorganisms. In the seminar, a new methanotroph from a wetland, showing respiratory oxidization of sulfur compounds will be presented. We also experimentally demonstrated that thiotrophy and methanotrophy are metabolically compatible, and both metabolisms can be expressed simultaneously in a single microorganism. These findings suggest the mixotrophic bacterium as a possible component of the methane and sulfur cycles in wetlands and set a new framework for better understanding methane and sulfur cycle interaction in natural and engineered wetlands.
It was found that methanotrophs of ‘Ca. Methylacidiphilum’ strains IT5 and IT6, derived from geothermal environments unexpectedly grew on C3 intermediates of propane oxidation as sole carbon and energy sources. The complete biochemical pathway for utilizing C3 substrates was predicted by genomic and transcriptomic analyses and supported by physiology and substrate specificity tests. Additionally, one of the isolates, strain IT6, respired a nitrogenous greenhouse gas, N2O, as an alternative electron acceptor in anoxic conditions. Thus, the discovery of a novel function of CuMMO, the ability to utilize C3 compounds and respire an alternative electron acceptor expand the current metabolic traits of methanotrophs.
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