In biological study, one sometimes needs to detect the simultaneous presence of a micro RNA (miRNA) and its putative target mRNA in the same cell.
As no such technique is unavailable, we develop a in situ method for this purpose. It uses rolling-circle amplification of RNAs to achieve a high signal/noise ratio.
It can be applied to simultaneous detection of multiple RNAs in the same cell.
Published in Plant Biotechnology Journal.